®
Bismillahirrahmanirrahim
SCIENCE
About CellO-IF
CellO-IF is a ready-to-use single reagent for immunofluorescence labeling of entire 2D/3D cell culture specimens, whole-mount organoids, spheroids, and co-culture models.
It's designed to streamline the immunofluorescence workflows to provide more data with better accuracy:
CellO-IF replaces multiple solutions, eliminating human error and variability caused by complex preparation. The formulation enables whole-sample labeling, providing the most accurate information possible.
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With CellO-IF workflow; organoids, spheroids and cells are visualized in their optimum growth environment, without any transfer, centrifuging, or tedious sectioning.
Here is a comparative list demonstrating the advantages of CellO-IF workflow over the traditional immunofluorescence workflows.
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Three liver cancer spheroids and surrounding cells were successfully labeled in situ using antibodies specific for Na-K ATPase (Green) and Albumin (Red). The nuclei are marked by DAPI (Blue). This video demonstrates the power of CellO-IF workflow to visualize cells directly within their hydrogel or any other growing environment, avoiding transfer or disruption.
01
WORKFLOW
Streamlined and Standardized Workflow:
8 steps, 1 reagent
CELLO-IF WORKFLOW
A Standardized IF labeling method to achieve more consistent results. CellO-IF is the all-in-one reagent that replaces multiple reagents in traditional workflows, eliminating preparation variability and human error. All you need are your primary and secondary antibodies, along with CellO-IF.
TRADITIONAL WORKFLOWS
Require multiple reagents (such as blocking, permeabilizing, and antigen-dilution solutions; reagents for harvesting, clearing, and antigen retrieval), which dramatically increase complexity, cost, and the risk of inconsistent results.
02
SAMPLE HANDLING
Preserves Sample Integrity:
Minimizes stress, damage, and loss of fragile cells, organoids, spheroids.
CELLO-IF WORKFLOW
Effortless: Labeling is performed in situ(while cells are still in its microenvironment like a hydrogel/ECM).
TRADITIONAL WORKFLOWS
Require harmful multiple steps like harvesting, clearing, transferring, centrifuging, paraffin embedding and sectioning.
03
SAMPLE LOSS
Higher Reproducibility & Reliability:
Protects valuable sample
CELLO-IF WORKFLOW
Minimal to None: No need for physical transfer/centrifugation steps.
TRADITIONAL WORKFLOWS
Significant risk of losing and damaging precious or small samples during multiple transfer and washing steps.
04
ANTIGENICITY
Enhanced Clarity & Detection:
Better high-resolution images and visualization of difficult targets.
CELLO-IF WORKFLOW
Boosted: Contains components that enhance antibody penetration and target visualization.
TRADITIONAL WORKFLOWS
Often require harsh permeabilization/clearing, which can compromise antigen structure.
05
BACKGROUND NOISE
Higher Signal-to-Noise Ratio:
Clearer, more accurate imaging.
CELLO-IF WORKFLOW
Reduced: Formulated to lower non-specific staining.
TRADITIONAL WORKFLOWS
High background can be a common issue, especially with 3D samples.
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APPLICABILITY
Enables High-Throughput Imaging:
Makes large-scale analysis of entire sample feasible.
CELLO-IF WORKFLOW
​Ideal for whole mount labeling of 3D organoids/spheroids and 2D cultures.
TRADITIONAL WORKFLOWS
Challenging for large, dense 3D structures; often requires tedious sectioning or complex clearing methods.
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COST
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ENVIRONMENTAL
IMPACT
Save Money, Reduce Waste:
Requires significantly less plastic consumables and minimizes waste.
CELLO-IF WORKFLOW
​Eliminates centrifugation, pipetting, and reagent preparation steps.
TRADITIONAL WORKFLOWS
High consumption of pipettes, tips, tubes, and labware due to extensive washing, transfer, and preparation steps.
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TIME TO RESULT
Faster Results:
Accelerates study timelines.
CELLO-IF WORKFLOW
Fast: Approximately 4-8 hours, depending on the sample (start to imaging).
TRADITIONAL WORKFLOWS
Slow: Can take ~44 hours or several days.
PUBLICATIONS
1-Demirel, G., Tok, O.E., Aktas, R.G. (2025). A Simplified and Robust Immunofluorescence Labeling Method for Complex 3D Cell Cultures: Minimizing Manipulation and Maximizing Data in Whole-Mount Analysis of Organoids, Spheroids, and Co-culture Models. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 2951. Humana, New York, NY. https://doi.org/10.1007/7651_2025_634
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2-Demirel, G., Cakıl, Y.D., Koltuk, G. et al. The use of hyaluronic acid in a 3D biomimetic scaffold supports spheroid formation and the culture of cancer stem cells. Sci Rep 14, 19560 (2024). https://doi.org/10.1038/s41598-024-69047-6
3-Tok OE, Demirel G, Saatci Y, Akbulut Z, Kayalar O, Aktas RG. Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel. J Vis Exp. 2022 Dec 23;(190). doi: 10.3791/64563. PMID: 36622008.
About CellO-M
CellO-M is a single, multipurpose container that eliminates the need for sample transfer by allowing sensitive cell models (such as organoids, spheroids, and stem cells) to be cultured, processed, frozen, and imaged in the same vessel, significantly reducing cell loss and experimental variability.
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Here is a comparative list demonstrating the advantages of CellO-M workflow over the currently available cell culture vessels .
Liver cancer spheroids were grown, coated, and imaged under a Scanning Electron Microscope (SEM) directly on CellO-M. This method eliminates the risk of sample loss or damage, enabling visualization of the entire sample for more data and better accuracy.
01
FUNCTION
All-in-One Multipurpose Container
CELLO-M
It is designed for culturing, processing, freezing, and imaging fragile samples in the same container and eliminates sample pipetting, centrifuging, and transfer steps.
STANDART WORKFLOWS
The user needs multiple single-step containers and consumables (e.g., centrifuge tubes, pipettes, cryogenic vials, multi-well plates).
02
SAMPLE HANDLING
Preservation of 3D Structure: Ensures samples remain intact for high-quality analysis.
CELLO-M
It is designed to prevent damage and loss of sensitive cells, organoids, and spheroids during the entire process.
STANDART WORKFLOWS
Traditional methods risk losing or damaging fragile samples, especially 3D models, during harsh centrifugation, washing, and transfer steps.
03
IMAGING
ANALYSIS
Optimized for Imaging
CELLO-M
It features a design that allows for direct, clear imaging of organoids spheroids.
STANDART WORKFLOWS
Suboptimal: Samples must often be moved to slides or specialized plates, which can disrupt the architecture and increase processing time.
04
WORKFLOW
EFFICIENCY
Saves Time and Labor: Accelerates experimental timelines, especially for high-throughput analysis.
CELLO-M
One container for multiple applications (culturing, processing, freezing and imaging under light, confocal, electron microscopes).
STANDART WORKFLOW
Requires manual transfer between several different types of labware (such as plates → tubes → vials
→slides, grids).
PUBLICATIONS
1-Demirel, G., Tok, O.E., Aktas, R.G. (2025). A Simplified and Robust Immunofluorescence Labeling Method for Complex 3D Cell Cultures: Minimizing Manipulation and Maximizing Data in Whole-Mount Analysis of Organoids, Spheroids, and Co-culture Models. In: Turksen, K. (eds) Organoids. Methods in Molecular Biology, vol 2951. Humana, New York, NY. https://doi.org/10.1007/7651_2025_634​
3-Tok OE, Demirel G, Saatci Y, Akbulut Z, Kayalar O, Aktas RG. Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel. J Vis Exp. 2022 Dec 23;(190). doi: 10.3791/64563. PMID: 36622008.