SCIENCE

A new method for immunofluorescent labeling of whole-mount organoids and spheroids while still in a hydrogel ​

• Use CellO™-IF, all-in-one reagent, for entire experiment

• Eliminate harvesting and clearing steps

• Bring simplicity, improve results

• Speed-up experiments

• Label up to three proteins within 6 hours

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• Grow organoids or spheroids in CellO™-M  and visualize viability and integrity

• Stain live cell membrane and nuclei within two hours

• Examine living 3D structures in their growing environment

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• Freeze and thaw organoids or spheroids while still in a hydrogel 

• Protect the 3D architecture of your sample              

• Stop  experiments by freezing the sample in CellO™-M when needed

• Restart experiments anytime by thawing your sample in CellO™-M and continue from where you left off

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• Stain organoids or spheroids with Hematoxylin & Eosin while still in a hydrogel.

• Visualize 3D growing structures and their surrounding environment with a simple and cost-effective method.

• Examine communications and connections between organoids or spheroids while they are still in their growing environment.

A simple method for immunohistochemistry of whole-mount organoids and spheroids while still in a hydrogel.

• Visualize the location of proteins while protecting the 3D architecture 

• Eliminate harmful steps in conventional methods, like antigen retrieving

• Use one solution, CellO™-P,  for all steps

• Speed-up the experiment 

• Generate spheroids in CellO™-M with a simple and fast method

• Examine development, interaction and invasion of living 3D growing structures

• Design co-culture experiments 

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• Investigate the 3D architecture of organoids or spheroids under a scanning electron microscope with an easy and user-friendly method 

• Use one CellO™-M to grow organoids or spheroids, then process and examine directly under a scanning electron microscope

• Examine your sample while still in its growing environment

• Eliminate transferring, pipetting, and harvesting steps

• Protect your entire sample

• Examine the ultrastructure of organoids or spheroids while still in the hydrogel

• Use one CellO™-M to grow organoids or spheroids, then process and prepare resin blocks for transmission electron microscopy 

• Save time

• Protect your entire sample

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• Speed-up and improve manuel immunostaining of paraffin sections with CellO™-P

• Eliminate harmful steps like antigen retrieval treatment

• Simplify experiment by using one solution

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